ExFLP-14 Determination of Extraneous Material in Pressed, Sliced, Chopped and Macerated Dates and Date Material
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BEE36127D93449A387DD62F679B9F162 |
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文件大小(MB)： |
0.27 |
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页数： |
7 |
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日期： |
2012-3-3 |
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Published by: POLYSCIENCE PUBLICATIONS, P.O. Box 1606, Station St-Martin, Laval, Quebec,Canada H7V 3P9. TEL.: 1-800-840-5870. FAX: (450) 688-1930.,Government of Canada Gouvernement du Canada,Laboratory Procedure ExFLP-14,September 1997,HEALTH PROTECTION BRANCH,OTTAWA,DETERMINATION OF EXTRANEOUS MATERIAL IN PROCESSED, SLICED,CHOPPED AND MACERATED DATES AND DATE MATERIAL,M.-A. Rivers,Research Division, Bureau of Microbial Hazards, Food Directorate,Health Canada, Ottawa, Ontario K1A 0L2,1. APPLICATION,This method is applicable to the sampling and examination of pressed, sliced, chopped, and macerated,dates and date material for insects, mites, hairs, feather barbules, sand, soil, pits, pieces of pit and other,extraneous material to determine compliance with Sections 4, 5 and 7 of the Food and Drugs Act. This,method replaces ExFLP-14, dated January 1986.,2. DEFINITION OF TERMS,A lot is defined as that amount (volume, weight, etc.) of dates or date material which is produced, stored,and/or shipped under conditions as nearly uniform as possible, consisting of not more than one variety, grade,or type of product from a single identifiable source.,3. COLLECTION OF SAMPLES,3.1 Scrutinize the entire lot for live infestation. If live infestation is found, do not sample until after,fumigation or other effective treatment.,3.2 Obtain three sample units selected at random from the lot of at least 200 g each using appropriate,sampling equipment and containers. Three sample units constitute a sample.,3.3 Each sample unit must be kept separate and labelled 1, 2, and 3. Complete information respecting,the lot size, weight of original containers, country of origin, exporter, importer and product and lot,identification should be recorded and should accompany the sample.,4. MATERIALS AND SPECIAL EQUIPMENT,1) Beakers (1 L),2) Smooth magnetic stirring bar, teflon coated (1 x 5 cm),3) Stirrer hot plate,4) U.S. standard sieve, No. 140,5) Wildman 2 L trap flask,6) Large funnel (~ 15.0 cm top diameter),7) Filtered or distilled water,ExFLP-14,September 1997,-2-,8) Wash bottles,9) Deodorized kerosene,10) Suction filtration apparatus. Use either a Hirsch or Buchner funnel. For both funnels the filter paper,(S&S #8 ruled) should be larger than the funnel plate so the paper forms a cup in the funnel. Moisten,the filter paper and make sure it gives good suction before filtering.,11) Clearing agent. A glycerol-alcohol (95%) mixture (1:1) is recommended.,12) Filter paper (S&S #8 ruled or equivalent),13) Petri dishes (10 cm),14) Alcohol. Use 95% ethanol or 99% isopropanol,15) Stereoscopic microscope (10-30x),16) Drying oven (40°-60°C)_,5. PROCEDURE,The examination shall be carried out in accordance with the following instructions.,5.1 Analytical Units,5.1.1 Break off portions of dates or date material in a random fashion from a sample unit and weight out,100 g. This 100 g amount constitutes an analytical unit.,5.1.2 Repeat Step 5.1.1 for each of the remaining two sample units.,5.2 Isolation : Light Filth,5.2.1 Transfer a 100 g analytical unit of dates or date material to a 1 L beaker.,5.2.2 Add 400 mL hot (50° - 70° C) tap water and a magnetic stir bar.,5.2.3 Bring mixture to come to a boil. For pressed dates boil 30-45 min with stirring until mixture is finely,dispersed. For sliced, chopped and macerated dates and date material boil for only 30 min with,magnetic stirring.,5.2.4 Transfer mixture to a No. 140 sieve and wet sieve with hot (50° - 70° C) tap water until the water runs,freely through the material and the discard water is clear.,5.2.5 Place the large funnel in the neck of the trap flask so the rod is coming through the funnel. Transfer,the material to the 2 L trap flask with a strong stream of water from a wash bottle. Bring volume to,900 mL with water.,5.2.6 Slowly add 50 mL of kerosene down trap rod.,5.2.7 Slowly bring stirrer to maximum speed that does not produce audible or visible splashing or a,permanent emulsion. All of the material should be in suspension and a portion of the stirring bar,should be visible at the bottom of the vortex. Stir magnetically 5 min.,5.2.8 Slowly add deaerated water down trap rod, maintaining top of stopper just above kerosene layer.,When stopper is almost fully raised, continue adding water until kerosene layer passes the stopper,and the bottom of kerosene layer is approximately 1 cm above top of fully raised stopper.,5.2.9 Swirl stopper through lower portion of trap flask so as to suspend settlings.,ExFLP-14,September 1997,-3-,5.2.10 Let mixture stand 30 min with intermittent stirring of bottom layer during first 20 min.,5.2.11 Spin stopper to remove sediment and ……
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